Principle of the procedure
This kit is based on colloidal gold immunochromatography and uses double-antigen sandwich assay to detect novel neutralizing antibodies against coronavirus in peripheral whole blood. The T line of the novel coronavirus neutralizing antibody test cassette was coated with recombinant novel coronavirus S-RBD antigen, and the C line was coated with sheep anti-S-RBD polyclonal antibody. During detection, the samples were dripped into the sample well, and the neutralizing antibodies of the novel coronavirus was combined with the recombinant S-RBD antigen of the novel coronavirus labeled with colloidal gold, a solid phase the recombinant S-RBD antigen of the novel coronavirus - novel coronavirus neutralizing antibodies - labelled the recombinant S-RBD antigen of the novel coronavirus - colloidal gold complex at the T line position, solid-phase sheep anti-S-RBD polyclonal antibody - the recombinant S-RBD antigen of the novel coronavirus-colloidal gold complex was formed at the C line.
| Application | In vitro semi-quantitative detection of neutralizing antibodies against SARS-CoV-2 in human serum, plasma, venous whole blood or peripheral whole blood with vaccination. |
| Main Composition | Test Cassette,Specimen Diluent, IFU |
| Application | Semi-quantitative detection of neutralizing antibodies against SARS-CoV-2 in human serum, plasma, venous whole blood or peripheral whole blood with vaccination. |
| Advantage | High specificity and high sensitivity |
| Test time | 15 minutes |
| Storage Condition | 2-30℃, out of directsunlight |
Test procedure
Please read the instruction for use carefully before testing, and complete the test in strict accordance with the directions of the manual, otherwise reliable results cannot be guaranteed.
1. Place the test cassette, sample diluent and test sample for 15~30 minutes, and equilibrate to room temperature (10~30℃).
2. Open the aluminum foil pouch of the test cassette, and place the test cassette on a flat surface.
3. Write sample ID on the plastic case of the test cassette.
4. Peripheral whole blood samples:
1). Tear off the disposable alcohol pad, wipe your finger, and dry naturally.
2). Using the lancet, pull out the lid of the lancet, press it on the finger that has been disinfected, and puncture the finger with the lancet.
3). Squeeze out the first drop of whole blood and wipe it off with a cotton swab.
4). Squeeze out the second drop of whole blood, and use a dropper to draw the whole blood above the mark.
5). Take 25μL sample (1 drop), add it into the sample well of the test cassette, and add 80μL(approximately 3 drops) of sample diluent. And then use a cotton swab to stop the bleeding.
6). Incubate for 15 minutes at room temperature. Result got after 30minutes is invalid.
5.Serum and plasma samples: Take 20μL sample(1 drop with a dropper), add it into the sample well of the test cassette, and add 80μL sample diluent (approximately 2~3 drops with a dropper). Incubate at 10~30℃for 15 minutes. Result got after 30 minutes is invalid.
6.Venous whole blood and peripheral whole blood samples: Take 25μL sample (1 drop with a dropper), add it into the sample well of the test cassette, and add 80 μ L sample diluent (approximately 2~3 drops with a dropper). Incubate at 10~30℃for 15 minutes. Result got after 30 minutes is invalid.
